Prepare Trypticase Soy Agar (M152). Sterilize as recommended and cool to 50°C. Add 5 ml defibrinated sheep blood to 100 ml agar. Mix and dispense 20 ml portions to 15 × 100 mm petri dishes. (Commercial trypticase soy-blood agar plates are satisfactory.)
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.