BAM Media M163: Tryptone Salt Agar (T1N1 Agar and T1N2 Agar)
|Trypticase or tryptone||10 g|
|Distilled water||1 liter|
Suspend ingredients and boil to dissolve agar. For slants, dispense into tubes. Autoclave 15 min at 121°C. Solidify tubes as slants. Cool medium for plates to 45-50°C and pour into sterile petri dishes.
For T1N2 agar, use 20 g NaCl rather than the 10 g specified for T1N1 agar.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.