FDA Grand Rounds: A Modular Approach for Enhanced Plasmid Subtyping and AMR Gene Profiling of Plasmids
February 9, 2023
- February 9, 2023
- 12:00 PM - 1:00 PM ET
About the Speaker
Lucas Harrison, Ph.D.
Division of Emerging Technologies
Office of Applied Science
Center for Veterinary Medicine (CVM)
Dr. Lucas Harrison is a Research Microbiologist in the Division of Emerging Technologies in the Office of Applied Science at the Center for Veterinary Medicine. Lucas studied mechanisms of antimicrobial tolerance and acquired antimicrobial resistance in Dr. Nancy Hanson’s lab while pursuing a Ph.D. in Medical Microbiology and Immunology at the Creighton University School of Medicine. He joined the Center for Veterinary Medicine in 2019 as a Staff Fellow where he evaluated genomic markers for source attribution in foodborne pathogens such as Salmonella, Campylobacter and E. coli. In his current position, he investigates the roles of antimicrobial resistance genes, virulence genes and plasmid diversity in foodborne pathogens.
About the Presentation
Plasmids are a major factor in the spread of antimicrobial resistance genes. Current plasmid typing methods do not account for the great degree of genetic diversity associated with the recombination mechanisms that allow plasmids to acquire antimicrobial resistance genes. In this presentation we introduce an enhanced plasmid subtyping method that not only identifies the genetic elements exclusive to and indicative of a plasmid type, but also characterizes plasmids based on the DNA sequence and relative position of these genetic elements as they are rearranged through plasmid recombination mechanisms.
- Describe how plasmids can bypass the host restriction of bacterial strains.
- Define the limitations associated with current plasmid typing methods.
- Discuss how the sequence and relative position of genetic elements can be used to differentiate plasmids, inform the composition of hybrid plasmids and indicate the location of prior recombination events.
Activity Outline FDA Grand Rounds: February 9, 2023